Data/hora: 16/05/2024 01:04:59

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	01/06/2020	
Data da última atualização: 	29/11/2021	
Autoria: 	RAMOS-GONZÁLEZ, P. L.; COSTA-RODRIGUES, M. da; POTSCLAM-BARRO, M.; CHABI-JESUS, C.; BANGUELA-CASTILLO, A.; HARAKAVA, RI.; KITAJIMA, E. W.; ASTUA, J. de F.	
Afiliação: 	PEDRO LUIS RAMOS-GONZÁLEZ, Instituto Biológico; MARIANE DA COSTA-RODRIGUES, Instituto Biológico; MATHEUS POTSCLAM-BARRO, Instituto Biológico; CAMILA CHABI-JESUS, Instituto Biológico; ALEXANDER BANGUELA-CASTILLO, Instituto Biológico; RICARDO HARAKAVA, Instituto Biológico; ELLIOT W. KITAJIMA, ESALQ; JULIANA DE FREITAS ASTUA, CNPMF.	
Título: 	First genome sequence of an isolate of hibiscus chlorotic ringspotvirus from the Western hemisphere.	
Ano de publicação: 	2020	
Fonte/Imprenta: 	Tropical Plant Pathology, 2020.	
Idioma: 	Inglês	
Conteúdo: 	For the first time, the near-complete genome sequence of the betacarmovirus, hibiscus chlorotic ringspot virus (HCRSV) isolated from the Americas is disclosed. High throughput sequencing of the total RNA extract from a Hibiscus-rosa sinensis (L.) plant collected in São Paulo state, Brazil, revealed the genome sequence of HCRSV isolate SBO1, which is 3945 nucleotides long and shows 93.1% nucleotide sequence identity with HCRSV_Singapore (X86448), considered the type member of the species. These two viruses display a similar genomic organization and potentially encode seven open reading frames (ORFs). In addition, a phylogenetic analysis based on a fragment with 557 nts of p38, the coat protein gene, from a cohort of 14 samples collected in Brazil revealed a no clearly defined segregation of South American isolates from those previously detected in geographical areas outside this continent. The practical consequences of the use of p38 ORF-derived amplicons for detection and variability studies of HCRSV are concisely discussed.	
Thesagro: 	Vírus.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
Exemplares: 	CNPMF - (33077-1) - UPC - AP - DD - Publicação digital -  -  - DIS